Sunday, 2 March 2025

IDENTIFICATION OF WHOLE MOUNT EMBRYO OF CHICK

 

Fig.1. Dorsal view ( X 14) of entire chick embryo having 4 pairs of mesodermic somites (about 24 hours incubation).

Identifying Characters:

  • Area Opaca & Area Pellucida quite distinct.
  • Primitive streak shortened to about half its original size & is restricted to the posterior end of area pellucida.
  • Presence of 1-4 somites, representing the segmented mesoderm.
  • Presence of head fold involving ectoderm & endoderm at the anterior tip of the head process.
  • Neural folds well developed towards anterior end.
  • Primitive streak in a reduced state. 
                   - Hence, the specimens seems to be a Chick embryo after 24hours of incubation.

Fig:2:Chick embryo after 48 hours of incubation.


Identifying Characters:

  • Neural tube is differentiated into brain & spinal cord.
  • Somites are 26 pairs.
  • S-shaped heart is seen to have turned onto the left side.
  • Presence of an enlarged telencephalon.
  • Optic cups are prominent.
                           -Hence,the specimen seems to be a Chick embryo after 48 hours of incubation.


Fig:3:Chick embryo after 72 hours of incubation.

Identifying Characters:
  • Paired cerebral hemisphere buldges  from the roof of telencephalon.
  • Somites are 35 pairs.
  • Appendages rudiments are present.
  • Optic cups with distinct,closed retina & lenses.
  • Head fold grows back & lies between 10-18 somites.
  • Tail bud is at 90degree angle to the axis of posterior trunk.
                   -Hence,the specimen seems to be a Chick embryo after 72 hours of incubation.

Fig:4:Chick embryo after 96 hours of incubation.



Identifying Characters:
  • Tail is segmented upto the tip of embryo.
  • Eye pigmentation is very distinct.
  • Caudal part of second arch distinctly elevated over surface.
  • Presence of more than 44 somites.
  • Length & width of wing bud & leg bud are almost equal.  
                -Hence,the specimen seems to be a Chick embryo after 96 hours of incubation.

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