Qualitaive Test for Carbohydrate: STARCH (CCF_CC2-P)

Sample No:5
	EXPERIMENT	OBSERVATION	INFERENCE
	Bi Uret Test
	3ml. of Sodium hydroxide soln.was added to 3 ml of supplied soln. & 2 drops of 1% CuSO4 reagent was added to it.	No Change	Absense of Proteins.
	Molisch’s Test
	2ml of sample solution is taken with 2 drops of 5% α-napthol or Thymol solution. Carefully inclined the tube & poured conc.H2SO4 drop wise, using a dropper, along sides of the tube.	Violet colour appeared at the junction of two liquids.	Presence of carbohydrate is confirmed.
	Iodine Test: (Indicator Test)
	2 drops of dilute Iodine reagent was added to 3 ml of supplied solution.	A deep blue coloration appeared	Presence of Starch is confirmed.

 [N.B.~Starch is a complex carbohydrate (polysaccharide) produced by most green plants as a primary means of energy storage. It primarily exists in two forms: amylose (a linear, helical chain of glucose) and amylopectin (a branched chain of glucose). ]

Qualitative Test of Carbohydrate: Maltose (CCF-CC2-P)

 

Sample No:3
	EXPERIMENT	OBSERVATION	INFERENCE
	Bi Uret Test
	3ml. of Sodium hydroxide soln.was added to 3 ml of supplied soln. & 2 drops of 1% CuSO4 reagent was added to it.	No Change	Absense of Proteins.
	Molisch’s Test
	2ml of sample solution is taken with 2 drops of 5% α-napthol or Thymol solution. Carefully inclined the tube & poured conc.H2SO4 drop wise, using a dropper, along sides of the tube.	Violet colour appeared at the junction of two liquids.	Presence of carbohydrate is confirmed.
	Iodine Test: (Indicator Test)
	2 drops of dilute Iodine reagent was added to 3 ml of supplied solution.	Deep blue coloration not appeared	Absence of Starch is confirmed.
	Seliwanoff’s Test
	1 ml. of supplied solution was added to 3 ml.of Seliwanoff’s reagent. Mixed thoroughly, boiled for 25 seconds and allowed to cool.	No red coloration in the solution.	Absence of  Sucrose.
	Benedict’s Test:
	5 drops of supplied solution was added to 3 ml Benedict’s qualitative reagent.The mixture was boiled for 2 minutes & cooled.	A brick red precipitate was obtained.	Presence of reducing sugar.
	Fehling’s Test:
	Solution"A" was mixed with solution"B" in 1:1. 2 ml of this mixture is added with few drops of the test sample to A test tube.Then it is placed in a water-bath at 60° C.	Red precipitate is obtained.	Presence of reducing sugar is confirmed.
	Barfoed’s Test: (Confirmatory Test)
	1 ml of supplied solution was added to 3 ml.of Barfoed’s reagent. The mixture was boiled for 30 sec and cooled.	No change.	Absence of Monosaccharides. Presence of Maltose is confirmed.

Qualitative Test for Carbohydrates: Sucrose (CCF_CC2_P)

 

Sample No:4
	EXPERIMENT	OBSERVATION	INFERENCE
	Bi Uret Test
	3ml. of Sodium hydroxide soln.was added to 3 ml of supplied soln. & 2 drops of 1% CuSO4 reagent was added to it.	No Change	Absense of Proteins.
	Molisch’s Test
	2ml of sample solution is taken with 2 drops of 5% α-napthol or Thymol solution. Carefully inclined the tube & poured conc.H2SO4 drop wise, using a dropper, along sides of the tube.	Violet colour appeared at the junction of two liquids.	Presence of carbohydrate is confirmed.
	Iodine Test: (Indicator Test)
	2 drops of dilute Iodine reagent was added to 3 ml of supplied solution.	Deep blue coloration not appeared	Absence of Starch is confirmed.
	Seliwanoff’s Test
	1 ml. of supplied solution was added to 3 ml.of Seliwanoff’s reagent. Mixed thoroughly, boiled for 25 seconds and allowed to cool.	A red coloration in the solution.	May be Sucrose.
	Hydrolysis Test: (Confirmatory Test)
	3 drops of Conc.H2SO4 was added to 5 ml of supplied soln.The mixture was boiled for 3 minutes. It was cooled and neutralized with solid Na2CO3 powder until no further effervescence occur.5 drops of this solution was added to 3 ml. of Benedict qualitative reagent. Mixture was boiled for 2 minutes and cooled.	A brick red precipitate was obtained.	Hence,presence of Sucrose is confirmed.

Qualitative Test for Carbohydrate : Fructose (CCF_CC2_P)

Sample No:2
	EXPERIMENT	OBSERVATION	INFERENCE
	Bi Uret Test
	3ml. of Sodium hydroxide soln.was added to 3 ml of supplied soln. & 2 drops of 1% CuSO4 reagent was added to it.	No Change	Absense of Proteins.
	Molisch’s Test
	2ml of sample solution is taken with 2 drops of 5% α-napthol or Thymol solution. Carefully inclined the tube & poured conc.H2SO4 drop wise, using a dropper, along sides of the tube.	Violet colour appeared at the junction of two liquids.	Presence of carbohydrate is confirmed.
	Iodine Test: (Indicator Test)
	2 drops of dilute Iodine reagent was added to 3 ml of supplied solution.	Deep blue coloration not appeared	Absence of Starch is confirmed.
	Benedict’s Test:
	5 drops of supplied solution was added to 3 ml Benedict’s qualitative reagent.The mixture was boiled for 2 minutes & cooled.	A brick red precipitate was obtained.	Presence of Reducing Sugar is confirmed.
	Fehling’s Test:
	Solution"A" was mixed with solution"B" in 1:1. 2 ml of this mixture is added with few drops of the test sample to A test tube. Then it is placed in a water-bath at 60° C.	Red Precipitate is obtained.	Presence of Reducing sugar is confirmed.
	Barfoed’s Test:
	1 ml of supplied solution was added to 3 ml. of Barfoed’s reagent. The mixture was boiled for 30 sec and cooled.	A red granular precipitate settled at the bottom of the testtube.	Presence of Monosaccharides.
	Seliwanoff’s Test: (Confirmatory Test)
	1ml. of supplied solution was added to 3 ml. of Seliwanoff’s reagent. Mixed thoroughly, boiled for 25 seconds and allowed to cool.	Red colour develops in the solution.	Presence of Keto Sugar. Presence of Fructose is confirmed.