Qualitative Test for Carbohydrate : Fructose (CCF_CC2_P)

Sample No:2
	EXPERIMENT	OBSERVATION	INFERENCE
	Bi Uret Test
	3ml. of Sodium hydroxide soln.was added to 3 ml of supplied soln. & 2 drops of 1% CuSO4 reagent was added to it.	No Change	Absense of Proteins.
	Molisch’s Test
	2ml of sample solution is taken with 2 drops of 5% α-napthol or Thymol solution. Carefully inclined the tube & poured conc.H2SO4 drop wise, using a dropper, along sides of the tube.	Violet colour appeared at the junction of two liquids.	Presence of carbohydrate is confirmed.
	Iodine Test: (Indicator Test)
	2 drops of dilute Iodine reagent was added to 3 ml of supplied solution.	Deep blue coloration not appeared	Absence of Starch is confirmed.
	Benedict’s Test:
	5 drops of supplied solution was added to 3 ml Benedict’s qualitative reagent.The mixture was boiled for 2 minutes & cooled.	A brick red precipitate was obtained.	Presence of Reducing Sugar is confirmed.
	Fehling’s Test:
	Solution"A" was mixed with solution"B" in 1:1. 2 ml of this mixture is added with few drops of the test sample to A test tube. Then it is placed in a water-bath at 60° C.	Red Precipitate is obtained.	Presence of Reducing sugar is confirmed.
	Barfoed’s Test:
	1 ml of supplied solution was added to 3 ml. of Barfoed’s reagent. The mixture was boiled for 30 sec and cooled.	A red granular precipitate settled at the bottom of the testtube.	Presence of Monosaccharides.
	Seliwanoff’s Test: (Confirmatory Test)
	1ml. of supplied solution was added to 3 ml. of Seliwanoff’s reagent. Mixed thoroughly, boiled for 25 seconds and allowed to cool.	Red colour develops in the solution.	Presence of Keto Sugar. Presence of Fructose is confirmed.