Qualitative Test for Carbohydrates: Sucrose (CCF_CC2_P)

 

Sample No:4
	EXPERIMENT	OBSERVATION	INFERENCE
	Bi Uret Test
	3ml. of Sodium hydroxide soln.was added to 3 ml of supplied soln. & 2 drops of 1% CuSO4 reagent was added to it.	No Change	Absense of Proteins.
	Molisch’s Test
	2ml of sample solution is taken with 2 drops of 5% α-napthol or Thymol solution. Carefully inclined the tube & poured conc.H2SO4 drop wise, using a dropper, along sides of the tube.	Violet colour appeared at the junction of two liquids.	Presence of carbohydrate is confirmed.
	Iodine Test: (Indicator Test)
	2 drops of dilute Iodine reagent was added to 3 ml of supplied solution.	Deep blue coloration not appeared	Absence of Starch is confirmed.
	Seliwanoff’s Test
	1 ml. of supplied solution was added to 3 ml.of Seliwanoff’s reagent. Mixed thoroughly, boiled for 25 seconds and allowed to cool.	A red coloration in the solution.	May be Sucrose.
	Hydrolysis Test: (Confirmatory Test)
	3 drops of Conc.H2SO4 was added to 5 ml of supplied soln.The mixture was boiled for 3 minutes. It was cooled and neutralized with solid Na2CO3 powder until no further effervescence occur.5 drops of this solution was added to 3 ml. of Benedict qualitative reagent. Mixture was boiled for 2 minutes and cooled.	A brick red precipitate was obtained.	Hence,presence of Sucrose is confirmed.