Identification of Entamoeba histolytica /CC6-P (B.SC.ZOOLOGY_MAJOR_CCF)

Identifying features are:

1. Body is more or less round in shape.
2. 20-40 µm in diameter.
3. Cytoplasm coarsely granulated.  Translucent ectoplasm & granular endoplasm are differentiated clearly.
4. Blade like Pseudopodium.
5. Presence of food vacuoles.
6. Nucleus is composed of delicate nuclear membrane covered by fine peripheral chromatin granules on the inner side & a central Karyosome.
7. Endoplasm contains red blood cells, leucocytes (occasionally),tissue debris & other cellular organelles.

         Hence, the specimen seems to be Trophozoite stage of Entamoeba histolytica.

1. Spherical, colourless body.
2.  10-20 µm in diameter with blunt pseudopodium.
3. A delicate nuclear membrane covered the nucleus. Nucleus internally covered by fine peripheral chromatin granules & a central karyosome.

       Hence, the specimen seems to be Pre-cystic stage of Entamoeba histolytica.

1. Two to Quadrinucleate, rounded form.
2. 7-9µm in diameter .
3. Clear & hyaline cytoplasm, contains black, rod like chromatoid bodies with round ends.
4.  Presence of 1-4 oblong bar like chromatoid bodies, with rounded ends.

       Hence, the specimen seems to be cystic stage of Entamoeba histolytica.

Systematic Position (Levine et al,1980)

Kingdom: Protozoa

 Phylum: Sarcomastigophora

       Sub Phylum: Sarcodina

           Super Class: Rhizopoda

              Class: Lobosea

                  Sub Class: Gymnamoebia

                        Order: Amoebida

                                Genus:Entamoeba

 Diagram:https://drive.google.com/file/d/12MJp8OiAP54HuL3jo3Xs5328kOC47Jqt/view?usp=sharing