IDENTIFICATION (with reason) OF Entamoeba sp. (B.Sc. ZOOLOGY_CCF-CC5_SEM4)

Identifying Characters are:

1. Microscopic, unicellular eukaryotic organism.

2. Irregular or definite body shape.

3. Locomotory organelles pseudopodia,cilia or flagella.

                   Hence, the specimen belongs to the Kingdom: Protozoa. 

1. Monomorphic type of nucleus.
2. Presence of flagella, Pseudopodia or both.
3. No spore formation.
4. Sexual reproduction, if present, essentially syngamy.

             Hence,the specimen belongs to the Phylum: Sarcomastigophora.

1. Naked body with external or internal test or skeleton.
2. Organ of locomotion pseudopodia.
3. Asexual reproduction by fission, sexuality, if present, associated with flagellate or more rarely amoeboid gametes.
4. Free living.

               Hence,the specimen belongs to the Sub Phylum: Sarcodina

1. Creeping form.
2. Locomotion by pseudopodia formation.

              Hence, the specimen belongs to the Super Class: Rhizopoda.

1. Pseudopodia lobose or more or less filiform type.
2. Generally uninucleate, but multinucleate forms also found.

              Hence, the  specimen belongs to Class: Lobosea

1. Body is more or less round in shape.
2. 20-40 µm in diameter.
3. Cytoplasm coarsely granulated.  Translucent ectoplasm & granular endoplasm are differentiated clearly.
4. Blade like Pseudopodium.
5. Presence of food vacuoles.
6. Nucleus is composed of delicate nuclear membrane covered by fine peripheral chromatin granules on the inner side & a central Karyosome.
7. Endoplasm contains red blood cells, leucocytes (occasionally),tissue debris & other cellular organelles.

         Hence, the specimen seems to be Trophozoite stage of Entamoeba histolytica.

1. Two to Quadrinucleate, rounded form.
2. 7-9µm in diameter .
3. Clear & hyaline cytoplasm, contains black, rod like chromatoid bodies with round ends.
4.  Presence of 1-4 oblong bar like chromatoid bodies, with rounded ends.

       Hence, the specimen seems to be cystic stage of Entamoeba histolytica.

Systematic Position (Levine et al,1980)

Kingdom: Protozoa

  Phylum: Sarcomastigophora

       Sub Phylum: Sarcodina

           Super Class: Rhizopoda

               Class: Lobosea

                  Sub Class: Gymnamoebia

                        Order: Amoebida

                                Genus:Entamoeba

 Diagram:https://drive.google.com/file/d/12MJp8OiAP54HuL3jo3Xs5328kOC47Jqt/view?usp=sharing