Qualitative Test of Carbohydrate: Maltose (CCF-CC2-P)

 

Sample No:3
	EXPERIMENT	OBSERVATION	INFERENCE
	Bi Uret Test
	3ml. of Sodium hydroxide soln.was added to 3 ml of supplied soln. & 2 drops of 1% CuSO4 reagent was added to it.	No Change	Absense of Proteins.
	Molisch’s Test
	2ml of sample solution is taken with 2 drops of 5% α-napthol or Thymol solution. Carefully inclined the tube & poured conc.H2SO4 drop wise, using a dropper, along sides of the tube.	Violet colour appeared at the junction of two liquids.	Presence of carbohydrate is confirmed.
	Iodine Test: (Indicator Test)
	2 drops of dilute Iodine reagent was added to 3 ml of supplied solution.	Deep blue coloration not appeared	Absence of Starch is confirmed.
	Seliwanoff’s Test
	1 ml. of supplied solution was added to 3 ml.of Seliwanoff’s reagent. Mixed thoroughly, boiled for 25 seconds and allowed to cool.	No red coloration in the solution.	Absence of  Sucrose.
	Benedict’s Test:
	5 drops of supplied solution was added to 3 ml Benedict’s qualitative reagent.The mixture was boiled for 2 minutes & cooled.	A brick red precipitate was obtained.	Presence of reducing sugar.
	Fehling’s Test:
	Solution"A" was mixed with solution"B" in 1:1. 2 ml of this mixture is added with few drops of the test sample to A test tube.Then it is placed in a water-bath at 60° C.	Red precipitate is obtained.	Presence of reducing sugar is confirmed.
	Barfoed’s Test: (Confirmatory Test)
	1 ml of supplied solution was added to 3 ml.of Barfoed’s reagent. The mixture was boiled for 30 sec and cooled.	No change.	Absence of Monosaccharides. Presence of Maltose is confirmed.